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Fluorescence-Based Assay for Demethylation
Detailed Description
WSU researchers have developed a fluorescence-based
assay for the dealkylation activity of the non-heme iron (II)
a-ketoglutarate-dependent dioxygenase family of enzymes. This assay uses
formaldehyde dehydrogenase to convert formaldehyde to formic acid and monitors
the creation of an NADH analog using fluorescence. The technology...
Published: 2/6/2014
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Inventor(s): Ashok Bhagwat, Todd Roy
Keywords(s):
Category(s): Assays, Life Science, Research tools
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