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Fluorescence-Based Assay for Demethylation

Detailed Description WSU researchers have developed a fluorescence-based assay for the dealkylation activity of the non-heme iron (II) a-ketoglutarate-dependent dioxygenase family of enzymes. This assay uses formaldehyde dehydrogenase to convert formaldehyde to formic acid and monitors the creation of an NADH analog using fluorescence. The technology...

Published: 2/6/2014 | Inventor(s): Ashok Bhagwat, Todd Roy

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Category(s): Assays, Life Science, Research tools